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SERS as a non-destructive technology provides chemical information in aqueous environments. Gold and silver are the most commonly applied materials for SERS substrates, as they offer high field enhancement in the visible to near infrared range due to their high density of electrons 16. Thus, hot spots have critical importance when designing a SERS nanostructure. Moreover, this enhancement can be further increased up to 10 15 orders of magnitude to SERS signal by hot spots 15, which are caused by the plasmonic coupling of the particles when they are very close to each other 14. In general, average enhancement factors for typical SERS substrates are amplified by 10 6 to 10 8 orders of magnitude comparing with their Raman signature 14. SERS signals can be collected from the molecules which are in close proximity of nanometallic surfaces with confined LSPR 13.

Surface enhanced Raman spectroscopy (SERS) also depends on plasmonic platforms 12.

Based on their optical properties, flexible functionalized nanomaterials have already been applied in sensing varieties of biomolecules, including biomarkers for cancer 8, enzyme 9, DNA 10, and other biological species 11. This LSPR phenomenon limits nanomaterials absorbing specific region of light, and makes the nanomaterials sensitive to the modifications of physical properties of nanomaterials and their environments showing plasmonic absorption shifts 5, 6, 7. This diagnosis field takes benefits from the design and synthesis of nanomaterials 2, 3, 4, especially noble metal nanomaterials showing localized surface plasmon resonance (LSPR) properties. Nanosystems has been widely developed in sensor devices for diagnostics, in vitro and in vivo diagnosis 1.

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